PELP1 Antibody

ProSci
Product Code: PSI-18-985
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-985-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PELP1 antibody (18-985) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunofluorescence analysis of L929 cells using PELP1 Polyclonal Antibody (18-985) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of U-2 OS cells using PELP1 Polyclonal Antibody (18-985) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PELP1 antibody (18-985) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of L929 cells using PELP1 Polyclonal Antibody (18-985) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using PELP1 Polyclonal Antibody (18-985) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Proline-, glutamic acid- and leucine-rich protein 1, Modulator of non-genomic activity of estrogen receptor, Transcription factor HMX3, PELP1, HMX3, MNAR
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1180 of human PELP1 (NP_055204.3).
NCBI Gene ID #:
27043
NCBI Official Name:
Proline-, glutamic acid- and leucine-rich protein 1
NCBI Official Symbol:
PELP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 160kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q8IZL8
User NOte:
Optimal dilutions for each application to be determined by the researcher.