UBC Antibody

ProSci
Product Code: PSI-18-994
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-994-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using UBC antibody (18-994) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human breast carcinoma using UBC antibody (18-994).
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Immunohistochemistry of paraffin-embedded human liver using UBC antibody (18-994) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using UBC antibody (18-994) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using UBC antibody (18-994) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human breast carcinoma using UBC antibody (18-994).
Immunohistochemistry of paraffin-embedded human liver using UBC antibody (18-994) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using UBC antibody (18-994) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Polyubiquitin-C, Ubiquitin, UBC
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBC (NP_066289.3).
NCBI Gene ID #:
7316
NCBI Official Name:
Polyubiquitin-C
NCBI Official Symbol:
UBC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kD/25kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
P0CG48
User NOte:
Optimal dilutions for each application to be determined by the researcher.