CAMK2G Antibody

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ProSci
Product Code: PSI-19-002
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-19-002-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CAMK2G antibody (19-002) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human stomach using CAMK2G antibody (19-002) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using CAMK2G antibody (19-002) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using CAMK2G antibody (19-002) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using CAMK2G Polyclonal Antibody (19-002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CAMK2G antibody (19-002) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using CAMK2G antibody (19-002) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CAMK2G antibody (19-002) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CAMK2G antibody (19-002) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CAMK2G Polyclonal Antibody (19-002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CaMK-II subunit gamma, CAMK2G, CAMK, CAMK-II, CAMKG
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human CAMK2G (NP_751909.1).
NCBI Gene ID #:
818
NCBI Official Name:
Calcium/calmodulin-dependent protein kinase type II subunit gamma
NCBI Official Symbol:
CAMK2G
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa/80kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
Q13555
User NOte:
Optimal dilutions for each application to be determined by the researcher.