TWIST1 Antibody

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ProSci
Product Code: PSI-19-004
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-004-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TWIST1 antibody (19-004) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1min.
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Immunohistochemistry of paraffin-embedded human tonsil using TWIST1 antibody (19-004) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using TWIST1 antibody (19-004) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using TWIST1 antibody (19-004) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1min.
Immunohistochemistry of paraffin-embedded human tonsil using TWIST1 antibody (19-004) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TWIST1 antibody (19-004) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
TWIST1, CRS, SCS, ACS3, CRS1, BPES2, BPES3, TWIST, bHLHa38
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TWIST1 (NP_000465.1).
NCBI Gene ID #:
7291
NCBI Official Name:
twist family bHLH transcription factor 1
NCBI Official Symbol:
TWIST1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 21kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Stem Cell, Transcription
Swissprot #:
Q15672
User NOte:
Optimal dilutions for each application to be determined by the researcher.