RBM17 Antibody

ProSci
Product Code: PSI-19-006
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-006-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RBM17 antibody (19-006) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded rat lung using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human endometrium cancer using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using RBM17 antibody (19-006) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat lung using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human endometrium cancer using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using RBM17 antibody (19-006) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
SPF45, splicing factor 45, 45 kDa-splicing factor, splicing factor 45kDa
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RBM17 (NP_116294.1).
NCBI Gene ID #:
84991
NCBI Official Name:
RNA binding motif protein 17
NCBI Official Symbol:
RBM17
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q96I25
User NOte:
Optimal dilutions for each application to be determined by the researcher.