PAK1 Antibody

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ProSci
Product Code: PSI-19-016
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-016-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse kidney, using PAK1 antibody (19-016) at 1:700 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat spleen using PAK1 Antibody (19-016) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using PAK1 Antibody (19-016) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of mouse kidney, using PAK1 antibody (19-016) at 1:700 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat spleen using PAK1 Antibody (19-016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PAK1 Antibody (19-016) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
PAK1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), MGC130000, MGC130001, PAKalpha, p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
Application Note:
WB: 1:500 - 1:2000

IHC: 1:25 - 1:100
Background:
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human PAK1
NCBI Gene ID #:
5058
NCBI Official Name:
p21 protein (Cdc42/Rac)-activated kinase 1
NCBI Official Symbol:
PAK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 82kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
Q13153
User NOte:
Optimal dilutions for each application to be determined by the researcher.