LRPPRC Antibody

ProSci
Product Code: PSI-19-047
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-047-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using LRPPRC antibody (19-047) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using LRPPRC antibody (19-047) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using LRPPRC antibody (19-047) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using LRPPRC antibody (19-047) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using LRPPRC antibody (19-047) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using LRPPRC antibody (19-047) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human lung cancer using LRPPRC antibody (19-047) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using LRPPRC antibody (19-047) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using LRPPRC antibody (19-047) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using LRPPRC antibody (19-047) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Leucine-rich PPR motif-containing protein, mitochondrial, 130 kDa leucine-rich protein, LRP 130, GP130, LRPPRC, LRP130
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:100 - 1:200

IF: 1:100 - 1:200
Background:
This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1041-1394 of human LRPPRC (NP_573566.2).
NCBI Gene ID #:
10128
NCBI Official Name:
Leucine-rich PPR motif-containing protein, mitochondrial
NCBI Official Symbol:
LRPPRC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P42704
User NOte:
Optimal dilutions for each application to be determined by the researcher.