ATP8A1 Antibody

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ProSci
Product Code: PSI-19-049
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-049-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATP8A1 antibody (19-049) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using ATP8A1 antibody (19-049) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Documents

Further Information

Additional Names:
ATPASEII, ATPIA, ATPP2, phospholipid-transporting ATPase IA, ATPase II, ATPase class I type 8A member 1, ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1, P4-ATPase flippase complex alpha subunit ATP8A1, aminophospholipid translocase, chromaffin granule ATPase II, probable phospholipid-transporting ATPase IA
Application Note:
WB: 1:500 - 1:2000
Background:
The P-type adenosinetriphosphatases (P-type ATPases) are a family of proteins which use the free energy of ATP hydrolysis to drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily catalyzes transport of heavy metal ions. Another subfamily transports non-heavy metal ions (NMHI). The protein encoded by this gene is a member of the third subfamily of P-type ATPases and acts to transport amphipaths, such as phosphatidylserine. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 420-545 of human ATP8A1 (NP_001098999.1).
NCBI Gene ID #:
10396
NCBI Official Name:
ATPase phospholipid transporting 8A1
NCBI Official Symbol:
ATP8A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 170kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9Y2Q0
User NOte:
Optimal dilutions for each application to be determined by the researcher.