ATP6V1B2 Antibody

ProSci
Product Code: PSI-19-149
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-149-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATP6V1B2 Antibody (19-149) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of HeLa cells using ATP6V1B2 antibody (19-149) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ATP6V1B2 Antibody (19-149) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using ATP6V1B2 antibody (19-149) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ATP6V1B2, ATP6B1B2, ATP6B2, V-ATPase subunit B 2, Vma2, VATB, VPP3, HO57, V-ATPase B2 subunit
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 262-511 of human ATP6V1B2 (NP_001684.2).
NCBI Gene ID #:
526
NCBI Official Name:
ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
NCBI Official Symbol:
ATP6V1B2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
P21281
User NOte:
Optimal dilutions for each application to be determined by the researcher.