ATP6V1E1 Antibody

ProSci
Product Code: PSI-19-150
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-150-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody (19-150) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.

Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody (19-150) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.

Further Information

Additional Names:
ARCL2C, ATP6E, ATP6E2, ATP6V1E, P31, Vma4, V-type proton ATPase subunit E 1, ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1, H(+)-transporting two-sector ATPase, 31kDa subunit, H+-transporting ATP synthase chain E, vacuolar, V-ATPase 31 kDa subunit, V-ATPase subunit E 1, V-ATPase, subunit E, vacuolar proton pump subunit E 1
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 77-226 of human ATP6V1E1 (NP_001687.1).
NCBI Gene ID #:
529
NCBI Official Name:
ATPase H+ transporting V1 subunit E1
NCBI Official Symbol:
ATP6V1E1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P36543
User NOte:
Optimal dilutions for each application to be determined by the researcher.