ENTPD1 Antibody

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ProSci
Product Code: PSI-19-156
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-19-156-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using ENTPD1 antibody (19-156) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 4
Immunofluorescence analysis of Raw264.7 cells using ENTPD1 Polyclonal Antibody (19-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of THP-1 cells using ENTPD1 Polyclonal Antibody (19-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of human placenta using ENTPD1 Polyclonal Antibody (19-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ENTPD1 antibody (19-156) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of Raw264.7 cells using ENTPD1 Polyclonal Antibody (19-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of THP-1 cells using ENTPD1 Polyclonal Antibody (19-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human placenta using ENTPD1 Polyclonal Antibody (19-156) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ATPDase, CD39, NTPDase-1, SPG64, ectonucleoside triphosphate diphosphohydrolase 1, CD39 antigen, ecto-ATP diphosphohydrolase 1, ecto-ATPDase 1, ecto-ATPase 1, ecto-apyrase, lymphoid cell activation antigen
Application Note:
WB: 1:200 - 1:1000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ENTPD1 (NP_001157655.1).
NCBI Gene ID #:
953
NCBI Official Name:
ectonucleoside triphosphate diphosphohydrolase 1
NCBI Official Symbol:
ENTPD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
P49961
User NOte:
Optimal dilutions for each application to be determined by the researcher.