CETN1 Antibody

ProSci
Product Code: PSI-19-160
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-160-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CETN1 antibody (19-160) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 2
Immunohistochemistry of paraffin-embedded human liver damage using CETN1 antibody (19-160) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CETN1 antibody (19-160) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver damage using CETN1 antibody (19-160) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CEN1, CETN, centrin-1, EF-hand protein, calcium binding protein, caltractin, centrin, EF-hand protein, 1, testicular tissue protein Li 37
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN1 (NP_004057.1).
NCBI Gene ID #:
1068
NCBI Official Name:
centrin 1
NCBI Official Symbol:
CETN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q12798
User NOte:
Optimal dilutions for each application to be determined by the researcher.