COX6A1 Antibody

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ProSci
Product Code: PSI-19-164
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-164-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using COX6A1 antibody (19-164) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat cerebellum using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using COX6A1 antibody (19-164) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat cerebellum using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Cytochrome c oxidase subunit 6A1, mitochondrial, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver, COX VIa-L, COX6A1, COX6AL
Application Note:
WB: 1:200 - 1:2000

IHC: 1:20 - 1:200

IF: 1:50 - 1:200
Background:
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-109 of human COX6A1 (NP_004364.2).
NCBI Gene ID #:
1337
NCBI Official Name:
Cytochrome c oxidase subunit 6A1, mitochondrial
NCBI Official Symbol:
COX6A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P12074
User NOte:
Optimal dilutions for each application to be determined by the researcher.