EIF4A2 Antibody

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ProSci
Product Code: PSI-19-185
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-185-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (19-185) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat brain using EIF4A2 antibody (19-185) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using EIF4A2 antibody (19-185) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using EIF4A2 antibody (19-185) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (19-185) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using EIF4A2 antibody (19-185) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using EIF4A2 antibody (19-185) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF4A2 antibody (19-185) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
EIF4A2, DDX2B, EIF4A, EIF4F, BM-010, eIF4A-II, eIF-4A-II
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2).
NCBI Gene ID #:
1974
NCBI Official Name:
eukaryotic translation initiation factor 4A2
NCBI Official Symbol:
EIF4A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q14240
User NOte:
Optimal dilutions for each application to be determined by the researcher.