ENO3 Antibody

ProSci
Product Code: PSI-19-186
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-186-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ENO3 antibody (19-186) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 2
Immunohistochemistry of paraffin-embedded rat testis using ENO3 antibody (19-186) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ENO3 antibody (19-186) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat testis using ENO3 antibody (19-186) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ENO3, MSE, GSD13
Application Note:
WB: 1:1000 - 1:4000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ENO3 (NP_001967.3).
NCBI Gene ID #:
2027
NCBI Official Name:
enolase 3 (beta, muscle)
NCBI Official Symbol:
ENO3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P13929
User NOte:
Optimal dilutions for each application to be determined by the researcher.