GALC Antibody

ProSci
Product Code: PSI-19-192
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-192-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GALC antibody (19-192) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver cancer using GALC antibody (19-192) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using GALC antibody (19-192) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using GALC antibody (19-192) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using GALC antibody (19-192) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using GALC antibody (19-192) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using GALC antibody (19-192) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GALC antibody (19-192) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
GALC,
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human GALC (NP_000144.2).
NCBI Gene ID #:
2581
NCBI Official Name:
galactosylceramidase
NCBI Official Symbol:
GALC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 77kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
P54803
User NOte:
Optimal dilutions for each application to be determined by the researcher.