GBP1 Antibody

ProSci
Product Code: PSI-19-195
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-195-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GBP1 antibody (19-195) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunohistochemistry of paraffin-embedded human liver cancer using GBP1 antibody (19-195) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of MCF7 cells using GBP1 antibody (19-195). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GBP1 antibody (19-195) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver cancer using GBP1 antibody (19-195) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using GBP1 antibody (19-195). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
GBP1, GBP-1, GTP-binding protein 1, Guanylate binding protein 1, HuGBP-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:20 - 1:200

IF: 1:10 - 1:100
Background:
Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 383-592 of human GBP1 (NP_002044.2).
NCBI Gene ID #:
2633
NCBI Official Name:
guanylate binding protein 1, interferon-inducible
NCBI Official Symbol:
GBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Chemokines & Cytokines, Immunology, Innate Immunity, Signal Transduction
Swissprot #:
P32455
User NOte:
Optimal dilutions for each application to be determined by the researcher.