GLS Antibody

ProSci
Product Code: PSI-19-197
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-197-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using GLS antibody (19-197) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach using GLS antibody (19-197) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse stomach using GLS antibody (19-197) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using GLS antibody (19-197) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using GLS antibody (19-197) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using GLS antibody (19-197) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human GLS (NP_055720.3).
NCBI Gene ID #:
2744
NCBI Official Name:
Glutaminase kidney isoform, mitochondrial
NCBI Official Symbol:
GLS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 69kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
O94925
User NOte:
Optimal dilutions for each application to be determined by the researcher.