HNRNPU Antibody

ProSci
Product Code: PSI-19-210
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-210-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HNRNPU antibody (19-210) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of C6 cells using HNRNPU antibody (19-210) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HNRNPU antibody (19-210) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of C6 cells using HNRNPU antibody (19-210) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
EIEE54, GRIP120-AS1, HNRPU, SAF-A, SAFA, U21.1, hnRNP U, pp12HNRNPU, heterogeneous nuclear ribonucleoprotein U, HNRNPU antisense RNA 1, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), nuclear p120 ribonucleoprotein, p120 nuclear protein
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 608-825 of human HNRNPU (NP_114032.2).
NCBI Gene ID #:
3192
NCBI Official Name:
heterogeneous nuclear ribonucleoprotein U
NCBI Official Symbol:
HNRNPU
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 120kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q00839
User NOte:
Optimal dilutions for each application to be determined by the researcher.