IFIT3 Antibody

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ProSci
Product Code: PSI-19-215
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-19-215-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using IFIT3 antibody (19-215) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human lung cancer using IFIT3 antibody (19-215) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver using IFIT3 antibody (19-215) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using IFIT3 antibody (19-215) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using IFIT3 antibody (19-215) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human lung cancer using IFIT3 antibody (19-215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using IFIT3 antibody (19-215) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using IFIT3 antibody (19-215) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
IFIT3 Antibody : P60, IRG2, IFI60, IFIT4, ISG60, RIG-G, CIG-49, GARG-49, Interferon-induced protein with tetratricopeptide repeats 3, CIG49, IFIT-3
Application Note:
WB: 1:500 - 1:1000

IHC: 1:100 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 128-331 of mouse IFIT3 (NP_034631.1).
NCBI Gene ID #:
15959
NCBI Official Name:
interferon-induced protein with tetratricopeptide repeats 3
NCBI Official Symbol:
IFIT3
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Chemokines & Cytokines, Immunology, Innate Immunity
Swissprot #:
O14879
User NOte:
Optimal dilutions for each application to be determined by the researcher.