INPP5B Antibody

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ProSci
Product Code: PSI-19-218
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-218-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using INPP5B antibody (19-218) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 3
Immunofluorescence analysis of C6 cells using INPP5B antibody (19-218) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using INPP5B antibody (19-218) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using INPP5B antibody (19-218) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of C6 cells using INPP5B antibody (19-218) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using INPP5B antibody (19-218) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Type II inositol 1,5-trisphosphate 5-phosphatase, 75 kDa inositol polyphosphate-5-phosphatase, Phosphoinositide 5-phosphatase, 5PTase, INPP5B, OCRL2
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human INPP5B (NP_005531.2).
NCBI Gene ID #:
3633
NCBI Official Name:
Type II inositol 1,4,5-trisphosphate 5-phosphatase
NCBI Official Symbol:
INPP5B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 113kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P32019
User NOte:
Optimal dilutions for each application to be determined by the researcher.