ITPKB Antibody

ProSci
Product Code: PSI-19-219
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-219-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ITPKB antibody (19-219) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human liver damage using ITPKB antibody (19-219) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using ITPKB antibody (19-219) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using ITPKB antibody (19-219) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ITPKB antibody (19-219) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver damage using ITPKB antibody (19-219) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ITPKB antibody (19-219) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ITPKB antibody (19-219) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Inositol-trisphosphate 3-kinase B, Inositol 1,5-trisphosphate 3-kinase B, IP3 3-kinase B, IP3K B, InsP 3-kinase B, ITPKB
Application Note:
WB: 1:200 - 1:2000

IHC: 1:20 - 1:200
Background:
The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ITPKB (NP_002212.3).
NCBI Gene ID #:
3707
NCBI Official Name:
Inositol-trisphosphate 3-kinase B
NCBI Official Symbol:
ITPKB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 102kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P27987
User NOte:
Optimal dilutions for each application to be determined by the researcher.