PMPCB Antibody

ProSci
Product Code: PSI-19-337
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-337-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PMPCB antibody (19-337) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human stomach using PMPCB antibody (19-337) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using PMPCB antibody (19-337) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using PMPCB antibody (19-337) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using PMPCB antibody (19-337) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using PMPCB antibody (19-337) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PMPCB antibody (19-337) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PMPCB antibody (19-337) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Mitochondrial-processing peptidase subunit beta, Beta-MPP, P-52, PMPCB, MPPB
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 110-380 of human PMPCB (NP_004270.2).
NCBI Gene ID #:
9512
NCBI Official Name:
Mitochondrial-processing peptidase subunit beta
NCBI Official Symbol:
PMPCB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology
Swissprot #:
O75439
User NOte:
Optimal dilutions for each application to be determined by the researcher.