DHX38 Antibody

ProSci
Product Code: PSI-19-349
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-349-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DHX38 antibody (19-349) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
2 / 2
Immunohistochemistry of paraffin-embedded human gastric cancer using DHX38 antibody (19-349) at dilution of 1:150 (40x lens).

Western blot analysis of extracts of various cell lines, using DHX38 antibody (19-349) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded human gastric cancer using DHX38 antibody (19-349) at dilution of 1:150 (40x lens).

Further Information

Additional Names:
DDX38, PRP16, PRPF16, RP84, pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16, ATP-dependent RNA helicase DHX38, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38, DEAH (Asp-Glu-Ala-His) box polypeptide 38, DEAH box protein 38, PRP16 homolog of S.cerevisiae
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:50 - 1:200
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1133-1227 of human DHX38 (NP_054722.2).
NCBI Gene ID #:
9785
NCBI Official Name:
DEAH-box helicase 38
NCBI Official Symbol:
DHX38
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 140kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q92620
User NOte:
Optimal dilutions for each application to be determined by the researcher.