EIF3M Antibody

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ProSci
Product Code: PSI-19-365
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-365-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EIF3M antibody (19-365) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human liver damage using EIF3M antibody (19-365) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using EIF3M antibody (19-365) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using EIF3M antibody (19-365) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human liver damage using EIF3M antibody (19-365) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EIF3M antibody (19-365) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
EIF3M, B5, FLJ29030, GA17, PCID1, hfl-B5, TANGO7
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a protein that is part of the eurkaryotic translation initiation factor 3 complete (eIF-3) required for protein synthesis. Elevated levels of the encoded protein are present in cancer cell lines. Inactivation of the encoded protein has been shown to interfere with translation of herpes virus mRNAs by preventing the association of mRNAs with the ribosomes. A pseudogene of this gene is located on the X chromosome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human EIF3M (NP_006351.2).
NCBI Gene ID #:
10480
NCBI Official Name:
eukaryotic translation initiation factor 3, subunit M
NCBI Official Symbol:
EIF3M
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q7L2H7
User NOte:
Optimal dilutions for each application to be determined by the researcher.