CRTAP Antibody

ProSci
Product Code: PSI-19-366
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-366-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of SW480 cells, using CRTAP antibody (19-366) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat lung using CRTAP antibody (19-366) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using CRTAP antibody (19-366) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using CRTAP antibody (19-366) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of SW480 cells, using CRTAP antibody (19-366) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using CRTAP antibody (19-366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using CRTAP antibody (19-366) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CRTAP antibody (19-366) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Cartilage-associated protein, CRTAP, CASP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is similar to the chicken and mouse CRTAP genes. The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli. Defects in this gene are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility and low bone mass.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human CRTAP (NP_006362.1).
NCBI Gene ID #:
10491
NCBI Official Name:
Cartilage-associated protein
NCBI Official Symbol:
CRTAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
O75718
User NOte:
Optimal dilutions for each application to be determined by the researcher.