DMC1 Antibody

ProSci
Product Code: PSI-19-393
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-393-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DMC1 antibody (19-393) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using DMC1 antibody (19-393) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.

Further Information

Additional Names:
DMC1H, LIM15, dJ199H16.1, meiotic recombination protein DMC1/LIM15 homolog, DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination, disrupted meiotic cDNA1, yeast, homolog of
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DMC1 (NP_008999.2).
NCBI Gene ID #:
11144
NCBI Official Name:
DNA meiotic recombinase 1
NCBI Official Symbol:
DMC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q14565
User NOte:
Optimal dilutions for each application to be determined by the researcher.