MRM3 Antibody

ProSci
Product Code: PSI-19-478
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-478-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MRM3 antibody (19-478) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using MRM3 antibody (19-478) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Further Information

Additional Names:
RMTL1, RNMTL1, rRNA methyltransferase 3, mitochondrial, 16S rRNA (guanosine(1370)-2'-O)-methyltransferase, 16S rRNA [Gm1370] 2'-O-methyltransferase, RNA methyltransferase like 1, RNA methyltransferase-like protein 1, putative RNA methyltransferase
Application Note:
WB: 1:500 - 1:2000
Background:
Efficient translation of mitochondrial-derived transcripts requires proper assembly of the large subunit of the mitochondrial ribosome. Central to the biogenesis of this large subunit is the A-loop of mitochondrial 16S rRNA, which is modified by three rRNA methyltransferases located near mtDNA nucleoids. The protein encoded by this gene methylates G(1370) of 16S rRNA, and this modification is necessary for proper ribosomal large subnit assembly. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of human MRM3 (NP_060616.1).
NCBI Gene ID #:
55178
NCBI Official Name:
mitochondrial rRNA methyltransferase 3
NCBI Official Symbol:
MRM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9HC36
User NOte:
Optimal dilutions for each application to be determined by the researcher.