NHEJ1 Antibody

ProSci
Product Code: PSI-19-514
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-514-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NHEJ1 antibody (19-514) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human breast cancer using NHEJ1 antibody (19-514) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterine cancer using NHEJ1 antibody (19-514) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using NHEJ1 antibody (19-514) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2OS cells using NHEJ1 antibody (19-514) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NHEJ1 antibody (19-514) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human breast cancer using NHEJ1 antibody (19-514) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using NHEJ1 antibody (19-514) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using NHEJ1 antibody (19-514) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2OS cells using NHEJ1 antibody (19-514) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
NHEJ1, nonhomologous end-joining factor 1, FLJ12610, XLF, Cernunnos, XRCC4-like factor
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1).
NCBI Gene ID #:
79840
NCBI Official Name:
nonhomologous end-joining factor 1
NCBI Official Symbol:
NHEJ1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9H9Q4
User NOte:
Optimal dilutions for each application to be determined by the researcher.