RRM2 Antibody

ProSci
Product Code: PSI-19-553
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-553-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using RRM2 antibody (19-553) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of A549 cells using RRM2 antibody (19-553). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug RRM2 antibody (19-553). Western blot was performed from the immunoprecipitate using RRM2 antibody (19-553) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using RRM2 antibody (19-553) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using RRM2 antibody (19-553). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug RRM2 antibody (19-553). Western blot was performed from the immunoprecipitate using RRM2 antibody (19-553) at a dilition of 1:1000.

Further Information

Additional Names:
RRM2, R2, RR2M, RR2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:10 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-389 of human RRM2 (NP_001025.1).
NCBI Gene ID #:
6241
NCBI Official Name:
ribonucleotide reductase M2
NCBI Official Symbol:
RRM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P31350
User NOte:
Optimal dilutions for each application to be determined by the researcher.