CD63 Antibody

ProSci
Product Code: PSI-19-556
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-556-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CD63 Antibody (19-556) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded rat brain using CD63 antibody (19-556) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using CD63 antibody (19-556) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using CD63 antibody (19-556) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CD63 Antibody (19-556) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using CD63 antibody (19-556) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CD63 antibody (19-556) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using CD63 antibody (19-556) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63 (NP_001771.1).
NCBI Gene ID #:
967
NCBI Official Name:
CD63 molecule
NCBI Official Symbol:
CD63
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 53kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology
Swissprot #:
P08962
User NOte:
Optimal dilutions for each application to be determined by the researcher.