DiMethyl-Histone H3-K14 pAb

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ProSci
Product Code: PSI-19-559
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-559-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K14 antibody (19-559).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 7
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K14 antibody (19-559) at 1:1000 dilution.
3 / 7
Immunohistochemistry of paraffin-embedded rat liver using DiMethyl-Histone H3-K14 antibody (19-559) at dilution of 1:200 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded human rectal cancer using DiMethyl-Histone H3-K14 antibody (19-559) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of C6 cells using DiMethyl-Histone H3-K14 pAb (19-559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using DiMethyl-Histone H3-K14 pAb (19-559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
7 / 7
Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 pAb (19-559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K14 antibody (19-559).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K14 antibody (19-559) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat liver using DiMethyl-Histone H3-K14 antibody (19-559) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using DiMethyl-Histone H3-K14 antibody (19-559) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using DiMethyl-Histone H3-K14 pAb (19-559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DiMethyl-Histone H3-K14 pAb (19-559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 pAb (19-559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
H3F3A, H3t, H3.4, H3/g, H3FT
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200

ChIP: 1:20 - 1:100

CHIP seq: 1:20 - 1:100
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human DiMethyl-Histone H3-K14
Modifications:
Methylated
NCBI Gene ID #:
8290
NCBI Official Name:
histone cluster 3, H3
NCBI Official Symbol:
H3K14me2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q16695
User NOte:
Optimal dilutions for each application to be determined by the researcher.