DDX5 Antibody

ProSci
Product Code: PSI-19-568
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-568-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DDX5 antibody (19-568) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 2
Immunofluorescence analysis of U2OS cells using DDX5 antibody (19-568). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DDX5 antibody (19-568) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of U2OS cells using DDX5 antibody (19-568). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DDX5, p68, HLR1, G17P1, HUMP68
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-614 of human DDX5 (NP_004387.1).
NCBI Gene ID #:
1655
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box helicase 5
NCBI Official Symbol:
DDX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 69kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P17844
User NOte:
Optimal dilutions for each application to be determined by the researcher.