RACGAP1 Antibody

ProSci
Product Code: PSI-19-569
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-569-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RACGAP1 antibody (19-569) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of U2OS cells using RACGAP1 antibody (19-569). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RACGAP1 antibody (19-569) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using RACGAP1 antibody (19-569). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RACGAP1, CYK4, ID-GAP, HsCYK-4, MgcRacGAP
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 353-632 of human RACGAP1 (NP_001119576.1).
NCBI Gene ID #:
29127
NCBI Official Name:
Rac GTPase activating protein 1
NCBI Official Symbol:
RACGAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 82kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Chemokines & Cytokines, Immunology, Signal Transduction
Swissprot #:
Q9H0H5
User NOte:
Optimal dilutions for each application to be determined by the researcher.