EFNA1 Antibody

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ProSci
Product Code: PSI-19-601
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-601-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EFNA1 antibody (19-601) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human prostate using EFNA1 Antibody (19-601) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using EFNA1 Antibody (19-601) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using EFNA1 Antibody (19-601) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using EFNA1 antibody (19-601) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human prostate using EFNA1 Antibody (19-601) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EFNA1 Antibody (19-601) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using EFNA1 Antibody (19-601) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
EFNA1, tumor necrosis factor, alpha-induced protein 4, TNFAIP4, ligand of eph-related kinase 1, LERK1, immediate early response protein B61, EPLG1, ephrin-A1, ephrin A1, eph-related receptor tyrosine kinase ligand 1, EFL1, ECKLG, B61
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-182 of human EFNA1 (NP_004419.2).
NCBI Gene ID #:
1942
NCBI Official Name:
ephrin-A1
NCBI Official Symbol:
EFNA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 21-23kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P20827
User NOte:
Optimal dilutions for each application to be determined by the researcher.