NEK2 Antibody

ProSci
Product Code: PSI-19-609
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-609-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NEK2 antibody (19-609) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human esophagus using NEK2 Antibody (19-609) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using NEK2 Antibody (19-609) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using NEK2 antibody (19-609). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NEK2 antibody (19-609) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human esophagus using NEK2 Antibody (19-609) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NEK2 Antibody (19-609) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NEK2 antibody (19-609). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
NEK2, NIMA-related kinase 2, NEK2A, HsPK21, NimA-like protein kinase 1, NLK1, HSPK 21, NimA-related protein kinase 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NEK2 (NP_001191112.1).
NCBI Gene ID #:
4751
NCBI Official Name:
NIMA-related kinase 2
NCBI Official Symbol:
NEK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P51955
User NOte:
Optimal dilutions for each application to be determined by the researcher.