PSMD9 Antibody

ProSci
Product Code: PSI-19-611
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-611-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using PSMD9 antibody (19-611) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
2 / 3
Immunohistochemistry of paraffin-embedded rat kidney using PSMD9 antibody (19-611) at dilution of 1:200 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using PSMD9 antibody (19-611). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PSMD9 antibody (19-611) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
Immunohistochemistry of paraffin-embedded rat kidney using PSMD9 antibody (19-611) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using PSMD9 antibody (19-611). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27, PSMD9
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human PSMD9 (NP_002804.2).
NCBI Gene ID #:
5715
NCBI Official Name:
26S proteasome non-ATPase regulatory subunit 9
NCBI Official Symbol:
PSMD9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
O00233
User NOte:
Optimal dilutions for each application to be determined by the researcher.