VAPB Antibody

ProSci
Product Code: PSI-19-617
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-617-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using VAPB antibody (19-617) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded human liver cancer using VAPB Antibody (19-617) at dilution of 1:200 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human kidney cancer using VAPB Antibody (19-617) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using VAPB Antibody (19-617) at dilution of 1:200 (40x lens).
5 / 5
Immunofluorescence analysis of HeLa cells using VAPB antibody (19-617). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using VAPB antibody (19-617) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using VAPB Antibody (19-617) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using VAPB Antibody (19-617) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using VAPB Antibody (19-617) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using VAPB antibody (19-617). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C, VAPB
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:50
Background:
The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 10-222 of human VAPB (NP_004729.1).
NCBI Gene ID #:
9217
NCBI Official Name:
Vesicle-associated membrane protein-associated protein B/C
NCBI Official Symbol:
VAPB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa/47kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
O95292
User NOte:
Optimal dilutions for each application to be determined by the researcher.