CHRM5 Antibody

ProSci
Product Code: PSI-19-620
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-620-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CHRM5 antibody (19-620) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
2 / 3
Immunohistochemistry of paraffin-embedded rat brain using CHRM5 antibody (19-620) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of U2OS cells using CHRM5 antibody (19-620). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CHRM5 antibody (19-620) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Immunohistochemistry of paraffin-embedded rat brain using CHRM5 antibody (19-620) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CHRM5 antibody (19-620). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HM5, muscarinic acetylcholine receptor M5, acetylcholine receptor, muscarinic 5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-430 of human CHRM5 (NP_036257.1).
NCBI Gene ID #:
1133
NCBI Official Name:
cholinergic receptor muscarinic 5
NCBI Official Symbol:
CHRM5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P08912
User NOte:
Optimal dilutions for each application to be determined by the researcher.