PA2G4 Antibody

ProSci
Product Code: PSI-19-625
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-625-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PA2G4 antibody (19-625) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded rat lung using PA2G4 Antibody (19-625) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using PA2G4 Antibody (19-625) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using PA2G4 Antibody (19-625) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using PA2G4 antibody (19-625). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PA2G4 antibody (19-625) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using PA2G4 Antibody (19-625) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using PA2G4 Antibody (19-625) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PA2G4 Antibody (19-625) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PA2G4 antibody (19-625). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PA2G4, EBP1, HG4-1, p38-2G4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-394 of human PA2G4 (NP_006182.2).
NCBI Gene ID #:
5036
NCBI Official Name:
proliferation-associated 2G4, 38kDa
NCBI Official Symbol:
PA2G4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9UQ80
User NOte:
Optimal dilutions for each application to be determined by the researcher.