BACH1 Antibody

ProSci
Product Code: PSI-19-636
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-636-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using BACH1 antibody (19-636) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat ovary using BACH1 antibody (19-636) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using BACH1 antibody (19-636) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using BACH1 antibody (19-636) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using BACH1 antibody (19-636) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using BACH1 antibody (19-636) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using BACH1 antibody (19-636) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using BACH1 antibody (19-636) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BACH1 antibody (19-636) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using BACH1 antibody (19-636) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
BACH1, BACH-1, AP000240.4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human BACH1 (NP_996749.1).
NCBI Gene ID #:
571
NCBI Official Name:
BTB and CNC homology 1, basic leucine zipper transcription factor 1
NCBI Official Symbol:
BACH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 115kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
O14867
User NOte:
Optimal dilutions for each application to be determined by the researcher.