CUL4B Antibody

ProSci
Product Code: PSI-19-640
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-640-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 7
Western blot analysis of extracts of various cell lines, using CUL4B antibody (19-640) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 7
Immunohistochemistry of paraffin-embedded rat liver using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded mouse kidney using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded mouse pancreas using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
5 / 7
Immunohistochemistry of paraffin-embedded human liver cancer using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
6 / 7
Immunofluorescence analysis of U2OS cells using CUL4B antibody (19-640). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
7 / 7
Immunofluorescence analysis of U2OS cells using CUL4B antibody (19-640).

Western blot analysis of extracts of various cell lines, using CUL4B antibody (19-640) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat liver using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using CUL4B antibody (19-640) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using CUL4B antibody (19-640). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
Immunofluorescence analysis of U2OS cells using CUL4B antibody (19-640).

Further Information

Additional Names:
Cullin-4B, CUL-4B, CUL4B, KIAA0695
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CUL4B (NP_003579.3).
NCBI Gene ID #:
8450
NCBI Official Name:
Cullin-4B
NCBI Official Symbol:
CUL4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 115kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q13620
User NOte:
Optimal dilutions for each application to be determined by the researcher.