DLD Antibody

ProSci
Product Code: PSI-19-643
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-643-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DLD antibody (19-643) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human thyroid cancer using DLD Antibody (19-643) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human thyroid cancer using DLD Antibody (19-643) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human endometrial cancer using DLD Antibody (19-643) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using DLD antibody (19-643) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human thyroid cancer using DLD Antibody (19-643) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using DLD Antibody (19-643) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human endometrial cancer using DLD Antibody (19-643) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
DLD, DLDH, E3, GCSL, LAD, PHE3, DLDD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 36-300 of human DLD (NP_000099.2).
NCBI Gene ID #:
1738
NCBI Official Name:
dihydrolipoamide dehydrogenase
NCBI Official Symbol:
DLD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P09622
User NOte:
Optimal dilutions for each application to be determined by the researcher.