EIF4B Antibody

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ProSci
Product Code: PSI-19-645
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-645-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EIF4B antibody (19-645) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human liver cancer using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using EIF4B antibody (19-645). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug EIF4B antibody (19-645). Western blot was performed from the immunoprecipitate using EIF4B antibody (19-645) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using EIF4B antibody (19-645) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human liver cancer using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EIF4B Antibody (19-645) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF4B antibody (19-645). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug EIF4B antibody (19-645). Western blot was performed from the immunoprecipitate using EIF4B antibody (19-645) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
EIF4B, EIF-4B, PRO1843
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
NCBI Gene ID #:
1975
NCBI Official Name:
eukaryotic translation initiation factor 4B
NCBI Official Symbol:
EIF4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P23588
User NOte:
Optimal dilutions for each application to be determined by the researcher.