MPG Antibody

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ProSci
Product Code: PSI-19-647
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-647-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MPG antibody (19-647) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 40s.
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Immunofluorescence analysis of U2OS cells using MPG antibody (19-647). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MPG antibody (19-647). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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Immunofluorescence analysis of A549 cells using MPG antibody (19-647).
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Western blot analysis of extracts of various cell lines, using MPG antibody (19-647) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 40s.
Immunofluorescence analysis of U2OS cells using MPG antibody (19-647). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MPG antibody (19-647). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence analysis of A549 cells using MPG antibody (19-647).

Documents

Further Information

Additional Names:
MPG, AAG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg, ADPG
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1).
NCBI Gene ID #:
4350
NCBI Official Name:
N-methylpurine-DNA glycosylase
NCBI Official Symbol:
MPG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P29372
User NOte:
Optimal dilutions for each application to be determined by the researcher.