STXBP1 Antibody

ProSci
Product Code: PSI-19-651
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-651-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using STXBP1 antibody (19-651) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat brain using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of rat brain using STXBP1 antibody (19-651) at dilution of 1:50. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse brain using STXBP1 antibody (19-651) at dilution of 1:50. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of HeLa cells using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using STXBP1 antibody (19-651) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat brain using STXBP1 antibody (19-651) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using STXBP1 antibody (19-651) at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using STXBP1 antibody (19-651) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
STXBP1, MUNC18-1, UNC18, hUNC18, rbSec1, P67, NSEC1, RBSEC1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 295-594 of human STXBP1 (NP_001027392.1).
NCBI Gene ID #:
6812
NCBI Official Name:
syntaxin binding protein 1
NCBI Official Symbol:
STXBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience
Swissprot #:
P61764
User NOte:
Optimal dilutions for each application to be determined by the researcher.