ORC6 Antibody

ProSci
Product Code: PSI-19-655
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-655-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ORC6 antibody (19-655) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of A549 cells using ORC6 antibody (19-655). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ORC6 antibody (19-655) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of A549 cells using ORC6 antibody (19-655). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ORC6L, origin recognition complex, subunit 6 homolog-like (yeast), ORC6, origin recognition complex subunit 6, subunit 6-like (yeast), subunit 6 (yeast homolog)-like, ORC6L
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human ORC6 (NP_055136.1).
NCBI Gene ID #:
23594
NCBI Official Name:
origin recognition complex, subunit 6
NCBI Official Symbol:
ORC6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y5N6
User NOte:
Optimal dilutions for each application to be determined by the researcher.