GARS Antibody

ProSci
Product Code: PSI-19-658
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-658-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GARS antibody (19-658) at 1:5000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded rat brain using GARS antibody (19-658) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using GARS antibody (19-658) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH/3T3 cells using GARS antibody (19-658) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GARS antibody (19-658) at 1:5000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using GARS antibody (19-658) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GARS antibody (19-658) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using GARS antibody (19-658) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CMT2D, DSMAV, GlyRS, HMN5, SMAD1, glycine--tRNA ligase, AP-4-A synthetase, Charcot-Marie-Tooth neuropathy 2D, Charcot-Marie-Tooth neuropathy, neuronal type, D, ap4A synthetase, diadenosine tetraphosphate synthetase
Application Note:
WB: 1:1000 - 1:4000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-370 of human GARS (NP_002038.2).
NCBI Gene ID #:
2617
NCBI Official Name:
glycyl-tRNA synthetase
NCBI Official Symbol:
GARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 83kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P41250
User NOte:
Optimal dilutions for each application to be determined by the researcher.