GLUL Antibody, KO Validated

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ProSci
Product Code: PSI-19-660
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-19-660-50uL50uL£505.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GLUL antibody (19-660) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and GLUL knockout (KO) HeLa cells, using GLUL antibody (19-660) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded human stomach using GLUL Antibody (19-660) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using GLUL Antibody (19-660) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using GLUL Antibody (19-660) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using GLUL antibody (19-660). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GLUL antibody (19-660) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and GLUL knockout (KO) HeLa cells, using GLUL antibody (19-660) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human stomach using GLUL Antibody (19-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using GLUL Antibody (19-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using GLUL Antibody (19-660) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GLUL antibody (19-660). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
GS, GLNS, PIG43, PIG59, GS
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GLUL (NP_001028228.1).
NCBI Gene ID #:
2752
NCBI Official Name:
glutamate-ammonia ligase
NCBI Official Symbol:
GLUL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P15104
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.