NQO2 Antibody

ProSci
Product Code: PSI-19-663
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-19-663-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of HeLa cells, using NQO2 antibody (19-663) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
2 / 2
Immunofluorescence analysis of U2OS cells using NQO2 antibody (19-663). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of HeLa cells, using NQO2 antibody (19-663) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using NQO2 antibody (19-663). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Ribosyldihydronicotinamide dehydrogenase [quinone], NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2, NQO2, NMOR2
Application Note:
WB: 1:500 - 1:2000

IF: 1:20 - 1:50
Background:
This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-231 of human NQO2 (NP_000895.2).
NCBI Gene ID #:
4835
NCBI Official Name:
Ribosyldihydronicotinamide dehydrogenase [quinone]
NCBI Official Symbol:
NQO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 26kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P16083
User NOte:
Optimal dilutions for each application to be determined by the researcher.